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Draw and Prepare Use a drawing needle to fill, then switch to a fresh injection needle (22-25G, 1-1.5")

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Optimal results require combining the peptides cellular effects with appropriate nutrition providing caloric deficit, regular cardiovascular exercise to oxidize mobilized fatty acids, and resistance training to preserve muscle mass during fat loss

Tablets allow consistent delivery of the same amount during each test cycle

Author response: We have altered the phrasing to read, Adult wildtype C57BL6/J mice were injected with TAM or matched dose (ml/kg) of vehicle (oil) for 5d. Reviewer 1 In regard to an issue raised by Reviewer 2 (below) I question whether the estrous cycle would need to be tracked