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The excitation wavelength of the JC-1 monomer was 488 nm, the exposure time was 300 ms, and the gain was 1.2

This oil was dissolved in Et 2 O (5.0 mL), cooled to 5 C., 1 M HCl in Et 2 O (12 mL, 12 mmol) was added dropwise while stirring to give a gum

In alveolar myofibroblasts, PRMT7 was shown to monomethylate histone H4 (H4R3me1) at the Forkhead box M1 (Foxm1) promoter, activating its transcription and promoting myofibroblast proliferation and elastin deposition

After transduction and selection using puromycin, colonies grown from single cell were picked and expanded for experiments

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