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1 Introduction Neutrophils are among the first responders in the innate immune response and are essential for pathogen clearance and host defense ( Interleukin-6 (IL-6) is an essential pro-inflammatory cytokine, necessary for an effective defense against pathogens, partially attributed to its role in stimulation of endothelial cells and lymphocytes ( A major limitation in studying concentration-dependent effects of IL-6 on the immune system is a lack of physiologically relevant experimental systems in which primary human neutrophil function can be tracked while controlling local IL-6 concentration

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the same medium was added to wells without cells