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Addition of any reducing agents was avoided

10.1016/j.resmic.2019.03.003

earlier studies with the double glycosylation of cysteine-containing peptides via installation of an alkyne at the Cys thiol using propargyl bromide, followed by sequential thiol-yne-thiol-ene couplings (Conte et al., Figure 6A) (Fiore et al., N and C terminus

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The authors hypothesized that this was due to trimetazidine affecting the brain tissue cell metabolism, and potentially transporting serotonin from brain tissue into the peripheral nervous system