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Culture media (DMEM/Ham F12 with HEPES and 4.5 g/L glucose), Dulbeccos phosphate-buffered saline (Dulbeccos PBS), Versene, antibiotics, and fetal calf serum (FCS) were purchased from Invitrogen

Thereafter, the sections were visualised using the BOND Polymer Refine Detection kit (DS9800, Leica Biosystems)

Similarly, in callus cultured on media containing Si-based materials, PPO activity was higher in the presence of 8 mg L -1 nano form (9.760.08 nM min -1 g -1 FW) and lower in bulk form at 4 mg L -1 (8.410.08 nM min -1 g -1 FW)

As the leading cause of chronic joint pain and functional disability, OA affects over 595 million individuals worldwide, with incidence rates escalating with aging populations and obesity epidemics [3, 4]

Chem Commun 53:68406843