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For whole-mount immunofluorescence and confocal imaging, organoids were fixed in 4% PFA for 60 minutes, pelleted (300x g for 5 min, low deceleration) and washed in PBS for 20 min, which was repeated three times

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Research involving GHK-Cu has examined collagen-related signaling, fibroblast activity, copper transport, inflammatory response, tissue remodeling, and gene-expression modulation