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2-10 nmol of L-glutamate standards and the cell lysate were mixed with reaction buffer (final concentrations of 36.8 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.1), 2.1910-5 U L-glutamate oxidase, 1 U peroxidase, 0.8 mM 3.5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulphopropyl) aniline (sodium salt) (DAOS), 0.8 mM aminoantipyrine)

The rats were 8 weeks old at the initiation of the studies with a body weight of 250325 g

Typically, weekly sessions are held initially, followed by bi-weekly or monthly maintenance sessions

Glutamate is also involved in psychiatric disorders because it is involved in the release of other neurotransmitters, including monoaminergic (e.g., serotonin and dopamine) and GABAergic transmission, which are often targets of psychiatric medications (4749)

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