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A single injection also carries less risk of side-effects, such as stomach irritation, than many oral anti-inflammatory medications

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Cells were lysed in 1.5 mL of RIPA buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) 100 for 30 min at 4 C