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Slides were then neutralized with 0.4 M TrisHCl, before being subjected to DNA staining using laboratory-specific staining protocols

Vitamin B12 can interact with medications such as colchicine (a medicine used to treat gout), metformin (an anti-diabetic agent), excessive alcohol, vitamin C products, extended release potassium products, antibiotics such as gentamicin, neomycin, tobramycin, anti-seizure medications such as phenobarbital, phenytoin, primidone, medicines used to treat heartburn like H2 blockers such as cimetidine and proton pump inhibitors such as omeprazole, antacids such as ranitidine, cimetidine

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The microsomes were incubated with 500 M solutions of Dihexa, piroxicam, verapamil, and 7-ethoxycoumarin, as controls for low, moderate, and highly metabolized compounds, respectively, in 0.1 M Tris buffer with an NADPH-regenerating system at 37C