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et al., 2018a)

Here we show that reduced dependence on exogenous glutamine is a generalizable feature of pluripotent stem cells

Taken together, it appears that mitochondria have co-opted the ISR as a local iron monitoring system, which can mark and eliminate organelles critically depleted for this essential metal

CRISPR-Cas9mediated gene knockout Target genes were knocked out via the Alt-R CRISPR-Cas9 system (Integrated DNA Technologies, Coralville, IA, USA) in combination with the Neon Nxt electroporation system (Thermo Fisher Scientific), following the manufacturers protocols