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Whole blood (20 mL) was layered upon a 15 mL Ficoll-Paque cushion (GE Healthcare, 17-1440-02) in 50 mL conical tubes, and centrifuged for 40 min at 400 x g at room temperature with the brake off

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used human corneal endothelial cells (HENC), stromal fibroblasts, and epithelial cells CEPI 17 CL 4 for bio-engineering human cornea construct (HCC) experiments, observing the permeability of diclofenac sodium-loaded SLNs on HCC

Sodium chloride - For some peptides (TB-500) such as TB-500 a 0.9% sodium chloride (saltwater) solution will work for reconstitution