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Two decades later, Kovalzon and Strekalova (2006) [6] were equally direct, calling DSIP a still unresolved riddle and noting that the connection between the peptide and sleep has never been further characterized, in part because of the lack of isolation of the DSIP gene, protein and possible related receptor. That framing has not materially changed in the literature since

The report's 4th tab takes into account all other modifications of the same sequence peptides and re-calculates relative abundances via normalization

The supernatant containing perchloric acid was neutralized using 4 M potassium hydroxide (KOH)

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