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While those medications target the GLP-1 (glucagon-like peptide-1) receptor, Tirzepatide is classified as a dual agonist, meaning it acts on both the GLP-1 receptor and the GIP (glucose-dependent insulinotropic polypeptide) receptor

Next, we employed a fluorescence polarization assay (FPA) to pinpoint the sequences crucial for binding among the specified regions, subsequently improving the cell applicability of the peptide sequence

Nature 468:214222

Immunofluorescence tissues were mounted with ProLong Gold (Life Technologies, Cat# P36934), while H&E and immunohistochemistry tissues were mounted with Permount mounting media (Fisher Scientific, Cat# SP15500) and coverslipped for imaging