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Description

Measurement of adenylates ATP, ADP, and AMP from intestinal and pancreatic cell lines, as specified in the legends, were analyzed by CE-MS as described previously, 157,174 with minor modifications

Consistent with the notion that glo-4 encodes a guanine nucleotide exchange factor for GLO-1, GLO-1::GFP marking was lost in glo-4 mutants (Figure 3H and 3I)

When ion translocation does not require direct ATP hydrolysis, this takes place upon subsequent reestablishment of the ionic gradients

Culture media were then removed and replaced with fresh growth medium with a fluorophore dye cocktail containing 100 nM MitoTracker Green FM, 50 nM MitoTracker Red CMXRos and 1 g/mL Hoechst 33342 (B2261, Sigma Aldrich, St