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Samples were taken at the start, and end of the incubation in the viscometer when the EI had reached a minimum value

Final purified proteins were stored in 20 mM Tris-HCl pH 8.0, 150 mM NaCl buffer, unless specified otherwise, and stored at 80 C until further use

Eur Heart J 36(38):25552564 van Riet EE et al (2014) Prevalence of unrecognized heart failure in older persons with shortness of breath on exertion

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