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Description

Med Phys 34(6):2371, 2007

3D spheroids were fixed with 4% paraformaldehyde/PBS for 45 min at 4 C, washed once with 0.1% Tween-20 in PBS, and suspended in wash buffer (SWB: 2% BSA, 0.1% Triton X-100, 0.025% SDS, 1 PBS)