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These tools were used to compute the binding energies using the MM-GBSA method, which involves two equations: The MM-GBSA free energy of binding (G bind ) is calculated as the difference between the free energy of the complex (G Complex ), and the sum of the free energies of the receptor (G Receptor ), and the ligand (G Ligand ), in solution ( Equation 2 represents the free energy of the molecule

In total the 83 sequences that clustered into 1 large Mu class GST group, alongside known Mu class GSTs, broadly demonstrated 19 clades of A

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