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Description

& Nadtochiy, S

Precipitated protein was resuspended in 4 M urea and 50 mM HEPES pH 7.4, followed by dilution to 1 M urea with the addition of 200 mM EPPS pH 8

Amino acids L-Alanine (AAA1580414), L-Cystine (AAA1376218), L-Glutamine (AAA1420118), L-Histidine (AAA1762718), L-Methionine (AAA1031822), L-Phenylalanine (AAA1323814), L-Proline (AAA1019914), L-Serine (AC132660250) and L-Tyrosine (AAA1114118) were obtained from Thermo Fisher