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[11] This variant has not been identified, but it has been suggested to coincide with a 22 kDa glycosylated variant of 23 kDa identified in the pituitary gland

However, in patients with pernicious anaemia these assays should not be used because the intrinsic factor antibodies in the patient's serum interferes with the binding of B12 to the intrinsic factor used in the assay, generating spurious results

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These defects were accompanied by disrupted phosphorylation of the IRS, as well as down-regulated expression of mitochondrial proteins PPAR co-activator 1 (PGC1) and uncoupling protein-2 (UCP-2) [200]

What this means practically is that your choice of morning versus evening should be driven by side effect management and routine compatibility, not by chasing marginally different blood levels