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The role of the glutathione antioxidant system in gut barrier failure in a rodent model of experimental necrotizing enterocolitis

Nonalcoholic steatohepatitis (NASH) patients have reduced levels of choline in the liver

Ghigo, E., Arvat, E., Gianotti, L., Imbimbo, B

Plasmid for single-stranded adeno-associated viral vectors 2/8 The plasmid pAAV-MCS-hAAT/EGFP was designed for maximum transgene expression and includes the hepatic locus control region from the ApoE gene (ApoE-HCR), a liver-specific 1-antitrypsin promoter (hAAT promoter), coagulation factor IX intron A (FIX intron A), a 3' mRNA transcription termination/polyadenylation signal (bghpA), and a reporter gene encoding enhanced green fluorescent protein (EGFP), as previously described (Miao et al

However, excessive accumulation of GSH precursors can overwhelm this reducing capacity, leading to reductive stress and aberrant mitochondrial H 2 O 2 production, a mechanism that is particularly pronounced in cancer cells characterized by elevated cysteine metabolism but limited antioxidant enzyme capacity 209