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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

Mitochondrial phospholipid hydroperoxide glutathione peroxidase suppresses apoptosis mediated by a mitochondrial death pathway

(Explained Simply) Your body naturally produces glutathione, a potent antioxidant

Longer than that, and you should evaluate whether the peptide is still viable

Following two washes with Perm/Wash and PBS/BSA solution, cells were resuspended in 80 l PBS/BSA and analyzed on an LSR-II flow cytometer with FACSDiva software (BD Biosciences)