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10.1016/j.tox.2020.152584 76 LiuY.PanR.OuyangY.GuW.XiaoT.YangH.et al (2024)

Zinc is an essential trace mineral that serves as a cofactor for over 300 enzymes and 1000 transcription factors, giving it important roles in immune function, sensory organ function, reproduction, gene regulation, DNA synthesis, wound healing, and the metabolism and activity of multiple other nutrients

observed that compared with the sham group, mice undergoing the AKI-to-CKD transition displayed decreased abundance of Actinobacteria and Firmicutes [169]

After the mixture was washed 5 times with a buffer composed of 50 mM HEPES-KOH (pH 7.5), 100 mM NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, 1 mM EDTA, and a protease inhibitor cocktail (EDTA-free), bound proteins were eluted with 0.1 M citrate and subjected to Western blotting with an anti-Kras antibody (1:250 dilution