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This revealed that both MTS-based cysteine synthesis and de novo GSH synthesis were continuously required to complement the TR/GR-null hepatocytes

The next day, cells were washed twice in pre-warmed Na + -free uptake buffer (137 mM choline chloride, 3 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 5 mM D-glucose, 0.7 mM K 2 HPO 4 , 10 mM HEPES, pH 7.4), then incubated for 10 minutes at 37 C

Some rats in the MMA and HMA groups were excluded due to accidental death during the experiment, which further reduced the final number of animals included in statistical analysis

In general, the decompensation of the aforementioned enzymes inhibits the scavenging of ROS, which is the root cause of redox imbalance in favor of pro-oxidants (43)

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