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The entire HPV-16 E7 amino acid sequence, reproduced as 12-mers with a 9 amino acid overlap, was synthesized on 30 different polypropylene rods

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Pelleted bacterial cells were lysed in 30 ml of lysis buffer (20 mM Tris-Cl (pH 7.5), 0.1% Triton X-100, 1 mg/ml lysozyme) for 20 min and sonicated, and insoluble molecules were removed

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pre-determined in the same assay in the absence of competing serum) to each well containing serially diluted pooled sera from each group, followed by a 1 h incubation at 37 C

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First-generation copper peptides, second-generation SRCP formulations, various ratios of peptide to copper, synthetic versus naturally occurring variants, each designed for specific applications that most guides fail to distinguish

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10.3390/pharmaceutics15020451 59 PuigM

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