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Enzymatic activity is determined by measuring the absorbance at 405 nm

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doi:10.1503/cmaj.181577

Construction of further hTrxR1 variants On the basis of the information provided by the crystal structure of the complex, 3 hTrxR1 mutants were produced from the hTrxR1 U498C mutant described above (hTrxR1 W114A,U498C , hTrxR1 N419A,U498C , and hTrxR1 W407A,N418/9A,U498C )

3.1 Lipid peroxidation One of the earliest and most significant effects of oxidative stress on spermatozoa is lipid peroxidation ( 2 ), interact with the PUFAs in sperm membranes