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Although the effect was not as pronounced as in a preventive model in Figure 4, sulfasalazine treatment from day 30 partially reversed the formation of TLS (Supplemental Figure 8, BD), with a corresponding decrease in the expression of TLS-related molecules (Supplemental Figure 8E)

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The zebrafish embryonic vascular system is highly conserved evolutionarily, and its functional circulatory system can be constructed within 24 hours after fertilization ( in vitro developmental characteristics of the embryo ( 2 Establishment of zebrafish breast cancer model Conventionally, genetically engineered murine (GEM) models and human cancer cell-derived xenograft models in immunodeficient strains (e.g., NOD-SCID) ( Zebrafish cancer models are constructed using three main strategies: genetic engineering modification, chemically induced carcinogenesis and xenograft models

doi: 10.1111/j.1365-2710.2009.01143.x