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Use a half-inch 27-30g needle to inject into the side of the penis avoiding visible veins

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Some of the right lungs were harvested and stored at -80C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining

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Selhub J, Jacques PF, Bostom AG, Wilson PW, Rosenberg IH