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In parallel, we performed a FACS-based CRISPR screen using a library of UPS-focused sgRNAs and a dual fluorescence stability reporter for the short isoform of BRD4, BRD4(S) (KBM7 cells expressing a BRD4(S)-TagBFP-P2A-mCherry construct) (Fig

Prior study showed that deficiency of Sentrin/SUMO-specific protease 2 (SENP2) resulted in hyper-SUMOylation of multiple potassium channels and neuronal hyperexcitability, finally triggering spontaneous seizures and sudden death [78, 79], which suggests that sumoylation is vital for epilepsy

Similarly, there are first examples for HOCl-based signaling: the extracellular action of HOCl in the induction of apoptosis in transformed cells has been termed the HOCl-signaling pathway (see [338] for a recent review)

This suggests that in the dataset analysed, reported GDP consistently varies sufficiently smoothly to be captured by a thin plate spline

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