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Description

coli BL21(DE3) using 0.5 mM IPTG induction at 37C for 6 h and purified initially using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva)

At lower levels of oxidant stress the final level of EI may not be reached within a practical timeframe, despite an excellent curve fit based on the final EI for each oxidant