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Positive immunostained cells were counted per five randomly visual fields in muscularis propria at 400 (Olympus FV500 optical microscope, Tokyo, Japan) by two observer who were blind to the study and presented as percentage of total area according to established methods

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Moreover, murine models have demonstrated that the pharmacologic or nutritional restoration of intestinal permeability significantly reduces alcohol-induced liver injury, confirming the causal role of microbial translocation [62,63]

[DOI] [PMC free article] [PubMed] [Google Scholar] 131.Yang J.-Y., Lee Y.-S., Kim Y., Lee S.-H., Ryu S., Fukuda S., Hase K., Yang C.-S., Lim H.S., Kim M.-S., et al

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