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To obtain permeabilized mitochondria, an aliquot of the intact mitochondrial preparation was diluted to 0.25 mg/ml, treated for 2 min with 20 g/ml alamethicin, pelleted at 14,000 g (2 min), and resuspended in the assay medium

In summary, this study demonstrates that calcium overload plays a critical role in the cytotoxicity associated with a-synucleinopathy, leading to mitochondrial damage, lysosomal dysfunction, and cell death

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162 Parathyroid hormone (PTH) and its therapeutic derivative teriparatide-a mainstay of bone anabolic therapy-have been shown to promote aerobic glycolysis in osteoblasts via the induction of IGF1, which in turn activates PI3K and mTORC2 163 (Fig