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After kinase reaction, phosphorylated GST-GSK3 protein was washed with phosphatase buffer (50 mM Tris-HCl (pH 7.4), 20 mM MgCl2, 0.2 mM EGTA, and 0.2 mM EDTA)

Regular treatments are recommended for optimal and long-lasting results

293 Additional studies on the pathological mechanism of depression have suggested that the HPA axis is hyperactive in MDD patients and that sensitivity to negative feedback is decreased

The randomized, double-blind, placebo-controlled trials were conducted over a period of 48 weeks and involved numerous intramuscular doses provided over several weeks and long-term safety monitoring was performed until week 74

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