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Based on the single-factor results, a three-factor, four-level orthogonal design was subsequently carried out with water content, fermentation time, and substrate loading as factors

Autophagy and Cardiometabolic Diseases: Academic Press

Different cell types have significantly different cystine uptake capacities, and cell types with low uptake capacity may exhibit a relatively high background

By measuring the ratio of heavy isotope-labelled peptides in urine samples using liquid chromatography (LC)-MS in multiple reaction monitoring (MRM) mode, we observed significantly higher Ggt1 levels in the OVX rats than in the control rats (Figure 3C, peptide determination results are shown in Supplementary Tables S9)

The filtrate is evaporated and the glutathione is purified by recrystallization from 50% ethanol