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Characterization of the S272A, D site-directed mutations of O-acetylserine sulfhydrylase: involvement of the pyridine ring in the alpha, beta-elimination reaction

The rest of this article is really about the second question

Nile Red, is a lipophilic, evil-like fluorescent probe that does not fluoresce in water and other polar solutions but appears as a bright red fluorescence in non-polar solvents, enabling the localization and quantification of intracellular lipids

Bronk L, Guan F, Ma D, Wang Y, Kerr M, Patel D, Titt U, Vassiliev O, Lin S, Mohan R , Grosshans D

Recently, the role of this cell death modality in the pathogenesis of various diseases has garnered increasing attention (epelak et al., 2020)