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The slides were then immersed in a lysis solution (2.5 M NaCl, 100 mM Na-ethylenediaminetetraacetic acid (EDTA), 10 mM Tris, 1% Triton X-100, and 10% DMSO, pH 10) for 1 h at 4 C and electrophoresed in an alkaline electrophoresis solution (300 mM NaOH, 10 mM Na-EDTA, pH 10) for 20 min at 4 C to allow DNA unwinding under alkaline/neutral conditions by incubating for 20 min

Schwarz, C., Benson, G

Ponnappan, U

To validate the effectiveness of the models predictions, we evaluated the impact of several gene targets associated with lysine synthesis ( lysC, thrA, metL, ppc, aspC , and panB ) on lysine production in E

Cases in the U.S