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High-dose biotin, also known as vitamin B7, can affect assays that use a biotin and streptavidin binding step, and the direction of the error depends on the assay format, with competitive designs prone to falsely high results and sandwich designs to falsely low ones

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On the other hand, the lack of a significant anxiolytic effect with GABA treatment raises questions about its bioavailability or receptor interactions in the specific context of the EPM test, possibly due to differences in the route of administration, dosage, or the animal models specific pathology