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The signaling pathway involving receptor activator of nuclear factor-B ligand (RANKL), its receptor (RANK), and osteoprotegerin (OPG) plays a pivotal role in the regulation of bone resorption

Liu R, Meng X, Yu X, Wang G, Dong Z, Zhou Z, et al

Distinctive signatures were also found in PNPLA3 rs738409, GPAM rs2792751, HSD17B13 rs4607179, and MTARC1 rs2642438

Targeted metabolomics by HPLC-MS/MS and FIA-MS/MS analysis Intracellular metabolites were extracted from tumors frozen in dry-ice cooled isopentane with 85:15 EtOH:PBS buffer and then sonicated 3 times for 5 s each, submerged in liquid nitrogen for 30 s, and thawed at 95 C twice