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Then, the abundance of the natural 13 C-labeled metabolite was subtracted from the total 13 C-labeled metabolite in MCF7 cells cultured with 13 C 3 -lactate

Neurological Function Score (Modified Longa System) Neurological deficits were evaluated 1 hour after ET-1 injection using the modified Longa scoring system: 0: No observable neurological deficit (excluded from analysis) 1: Failure to fully extend the left forepaw 2: Consistent circling to the left 3: Falling or leaning to the left side when ambulating 4: No spontaneous movement (severe infarction) Animals scoring between 1 and 3 were considered to have moderate ischemic deficits and were included in further analyses

Exposure to arsenic was determined from analyses of arsenic in drinking-water, performed by the state health laboratory between 1976 and 1997

The acetyl group is attached to serine at the 530 position in COX-1 and to serine at the 516 position in COX-2