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The supernatant containing the chromatin fragments was collected via centrifugation at 12,000 g for 10 min

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For instance, a meta-analysis of ABR studies in normal-hearing tinnitus patients found consistently elevated V/I ratios indicative of central gain enhancement, although inconsistencies in wave V amplitude changes suggest that gain alterations may be necessary for tinnitus generation but not for its maintenance (Chen et al., 2021)