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After being fed HFD for a total of 16-weeks (an appropriate rodent model of DIO and comparable to human obesity [21]) and reaching pre-arrival body weights (~38g), mice were randomized into balanced control and treatment cohorts (n=9/cohort), with similar group average body weight and standard deviation

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However, 18% of adults and 4% of children remain anemic even after long-term GFD, requiring further evaluation and iron supplementation

N., & Hansen, K