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CC cells were inoculated in 6-well plates at a density of 500 cells/well and routinely cultured for 2 weeks, fixed using 4% paraformaldehyde (PFA) for 20 min at RT, and stained with crystal violet (C0121, Beyotime) for 10 min before the clones were photographed and counted

Figure 5 Conclusions and perspectives The post-genomics era has witnessed a dramatic increase in our understanding of plant antioxidative systems

pH 7.0) to which the following solutions were added: 25 L of glutathione reductase solution (100 /mL), 25 L of sodium azide (0.12 m), 50 L Na 2 EDTA (0.15 mm), 50 L of NADPH (3 mm), and 50 L aliquots of supernatant obtained after centrifugation at 14,000 g for 25 min

Flow cytometric analysis of Lipid Peroxidation or ROS Cell lines Adherent cells were lifted using TrypLE (ThermoFisher Scientific, 12604013)