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Description

Am J Med 104(3):246-51, 1998

Reverse transcription reactions (20 l) were carried out with SuperScript III reverse transcriptase (Thermo Fisher, 18080085) according to the manufacturers instructions and containing 4 l 5x first-strand buffer, 1 l of murine RNase inhibitor (NEB, 174M0314S), 1 l of 10 mM dNTP mix (Thermo Fisher, 18427-013), 50 ng of random hexamers (Bioline, BIO-38028), and 1 g of total RNA (in a total volume of 11 l)

K.TiwariD

Fruitful developments have also occurred by accident

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