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This line was transfected with a plasmid encoding the mutant EGF receptor (variant vIII) and G418 resistance

Loss-of-function alterations in KEAP1 (or activating mutations in NRF2) allow tumor cells to hijack this pathway, leading to constitutive NRF2 activation, elevated antioxidant defenses (for example, GSH biosynthesis and detoxification enzymes) and enhanced tolerance to chemotherapy-induced oxidative injury 15

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