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Hcy is directly related to oxidative stress and inversely reflects B vitamin sufficiency, especially that of B6

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Colorimetric detection of GSH based on the pFeSAN In the standard procedure, the assays were performed in 1 mL HAc-NaAc buffer (0.1 M, pH 4.0) with different concentrations of GSH (0.05, 0.5, 2.5, 5, 12.5, 25, 50, 100, 150, 200, 250, 500, or 1000 M), where the TMB and pFeSAN concentrations were 1 mM and 20 g/mL, respectively

Both substances are required for normal growth and development, but the human body does not synthesize them