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However, the modified lysine limits the antimicrobial capacity toward overcoming the limitations of the Gram-negative outer membrane (Lukacik et al., 2012)

As a control, one of the infected precleared lysates was incubated on the beads without the primary antibody (no first antibody (NFA))

All amino acid sequences of GSTs were aligned using Multiline ( Multiple alignments were performed with the full-length amino acid sequences of the GST class proteins using MEGA 11 ( [33], and the same software was used to construct a phylogenetic tree based on the Maximum-likelihood algorithm with bootstrap analysis of 1,000 iterations