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Chen YC, Sheen JM, Hsu MH et al (2020) Melatonin rescued methotrexate-induced spatial deficit and hyperhomocysteinemia and increased asymmetric dimethylarginine in plasma and dorsal hippocampus in developing rats

This NO-loaded form of hemoglobin can release NO, which is then transferred to targets such as Band 3 on the erythrocyte membrane or to thiol-containing molecules in the endothelium (Su et al., 2020)

In that case, you need to find out the cause with medical advice

The K m values were remarkably similar ( 0.10.3 mM), but V max values were more varied