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The purified GR was incubated in the above buffers for 60 min at room temperature before measuring the activity at various pH conditions

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Conflict of interest: All authors: nothing to disclose [ [P865 Table] Bone marrow cell counts according to G-CSF priming] P866 Understanding collection efficiency and recovery in stimulated and non-stimulated blood- and bone marrow apheresis Jacqueline Overdevest, Patricia Spruitenburg, Jaap Jan Zwaginga, Tanja Netelenbos Leiden University Medical Center, Department of Immunohematology and Blood Transfusion, Leiden, Netherlands Background: In peripheral blood stem cell (PBSC) collection using an apheresis device, collection efficiency (CE) of mononuclear cells (MNC) is often calculated as an indicator for a successful procedure