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The conditions in which the sample is applied to the column are chosen considering the conditions which the interaction between analyte and ligand is strong, mostly resembling the natural conditions of the analyte and ligand

Furthermore, rodents do not easily develop addictive behaviour, naturally decrease alcohol consumption as acetaldehyde levels rise, metabolize alcohol quickly, have a high basal metabolic rate and demonstrate certain differences in the innate immune system 140

It is worth noting that the derivation of HBGVs applies extrapolation factors (also termed safety factors) to account for toxicodynamic and toxicokinetic differences between species and inter-individual variability in humans

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