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Benzyl alcohol was selected to refrain from decomposition of the final amino acids during the acid hydrolysis of, e.g., methyl esters [99] since for a mixture of 96 and 97 hydrogenolysis cleanly liberates (2 S ,3 S ,4 S )- 4 (Scheme 25) [100]

We used 360 l of an aqueous solution containing 50 mM NaP i buffer pH 7.1 and 30 mM DTT and placed it for 5 minutes in a water bath maintained at 43 C

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