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The inventors have found that in some embodiments the inclusion of an extraneous or exogenous thiol as an additive in the reaction mixture reduces the formation of undesirable by products

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For detection of O 2 , the sheaths were incubated in 6 mM NBT dissolved in PBS (pH 4.8) for infiltration

Kharitonov SA, Corradi M, van Rensen L et al (2000) Exhaled 8-isoprostane as a new non-invasive biomarker of oxidative stress in cystic fibrosis